Alternative Bacteriophage Display Systems

Authors: Castagnoli L.; Zuccconi A.; Quondam M.; Rossi M.; Vaccaro P.; Panni S.; Paoluzi S.; Santonico E.; Dente L.; Cesareni G.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 4, Number 2, April 2001 , pp. 121-133(13)

Publisher: Bentham Science Publishers

Abstract:

Filamentous phage has been extensively used to implement various aspects of phage display technology. The success of these organisms as vectors to present foreign peptides and to link them to their coding sequences is a consequence of their structural and biological characteristics. Some of these properties, however, represent a limitation when one attempts to display proteins that cannot be efficiently exported through the bacterial membrane or do not fold properly in the periplasm. Thus, the desirability of developing alternative display systems was recognised recently and led to the development of a different class of display vectors that assemble their capsid in the cytoplasm and are released via cell lysis. This review describes and compares the properties of these alternative display systems.

Keywords: Filamentous; SOC; HOC; T7 DISPLAY; gpV CARBOXY-TERMINAL; gpD AMINO-TERMINAL FUSION

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1386207013331174

Publication date: 2001-04-01

• Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.

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