Polymeric Liquid Membrane Electrodes Incorporated with Macrocyclic Hexaamines for Screening Adenine Nucleotides

Authors: Szymanska I.; Radecka H.; Radecki J.; Pietraszkiewicz M.; Pietraszkiewicz O.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 3, Number 6, December 2000 , pp. 509-517(9)

Publisher: Bentham Science Publishers

Abstract:

Lipophilic macrocyclic hexaamines supported by a poly(vinyl chloride) PVC matrix were used for the construction of liquid membrane electrodes sensitive toward adenine nucleotide polyanions. The membrane potential strongly depended on the pH of the sample solution. This phenomenon occurs due to the ability of the ionophore to accept protons. Therefore, the optimum pH was determined based on potential-pH profile. The potential measurements were carried out at pH 6.0 in the presence of 10-2 M 2-( N-morpholino) ethanesulfonic acid (MES) buffer. The potential response of these electrodes toward ATP-4 and/or HATP-3 was close to the Nernstian slope. The selectivities against ADP-3 , AMP-2 , HPO4-2 , and monovalent inorganic anions were estimated using the matched potential method. Chloride ions slightly affected potential response of the electrodes toward ATP-4 /HATP-3 . The influence of ionophore chemical structure on the selectivity and the sensitivity of these electrodes is briefly discussed.

Keywords: Polymeric Liquid Membrane; Screening Adenine Nuclotides; Lipophilic macrocyclic hexaamines; EMF responses; Electrode Response; Adenine Nucleotide Polyanions; Lipophilic macrocyclic pentaamine

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1386207003331436

Publication date: 2000-12-01

• Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.

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