Targeting of Phage Display Vectors to Mammalian Cells

Authors: Uppala A.; Koivunen E.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 3, Number 5, October 2000 , pp. 373-392(20)

Publisher: Bentham Science Publishers

Abstract:

Phage display libraries offer a strategy to isolate peptide ligands to target proteins and to define potential interaction sites between proteins. Recent studies have indicated a novel utility for phage display in that bacteriophage engineered to express peptide ligands to specific cell surface receptors are internalized by mammalian cells. Thus, reporter genes such as green fluorescent protein and lacZ harbored in the phage genome can be delivered to mammalian cells using targeting peptides displayed on the surface of phage. There is also the possibility to generate novel types of peptide libraries expressed intracellularly using a phage capable of inducing expression of its coding genes in human cells.

Keywords: Phage Display Libraries; LacZ; Gene Therapy; Cancer Therapy; Receptor Mediated Endocytosis; Filamentous Bacteriophage; Animal Viral Systems; Phage Capsid Proteins; Capsid Display Systems; pIII Expression; pVIII Expression; Gene Therapeutic Applications

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1386207003331517

Publication date: 2000-10-01

• Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.

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• By this author: Uppala A. ;  Koivunen E.

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