Targeting of Phage Display Vectors to Mammalian Cells

Authors: Uppala A.; Koivunen E.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 3, Number 5, October 2000 , pp. 373-392(20)

Publisher: Bentham Science Publishers

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Abstract:

Phage display libraries offer a strategy to isolate peptide ligands to target proteins and to define potential interaction sites between proteins. Recent studies have indicated a novel utility for phage display in that bacteriophage engineered to express peptide ligands to specific cell surface receptors are internalized by mammalian cells. Thus, reporter genes such as green fluorescent protein and lacZ harbored in the phage genome can be delivered to mammalian cells using targeting peptides displayed on the surface of phage. There is also the possibility to generate novel types of peptide libraries expressed intracellularly using a phage capable of inducing expression of its coding genes in human cells.

Keywords: Phage Display Libraries; LacZ; Gene Therapy; Cancer Therapy; Receptor Mediated Endocytosis; Filamentous Bacteriophage; Animal Viral Systems; Phage Capsid Proteins; Capsid Display Systems; pIII Expression; pVIII Expression; Gene Therapeutic Applications