Identification of Differential Gene Expression by High Throughput Analysis

Author: To K-Y.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 3, Number 3, June 2000 , pp. 235-241(7)

Publisher: Bentham Science Publishers

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Abstract:

High throughput analysis of differential gene expression is a powerful tool that can be applied to many areas in molecular cell biology, including differentiation, development, physiology, and pharmacology. In recent years, a variety of techniques have been developed to analyze differential gene expression, including comparative expressed sequence tag sequencing, differential display, representational difference analysis, cDNA or oligonucleotide arrays, and serial analysis of gene expression. This review explains the technologies, their scopes, impact on science, as well as their costs and possible limitations. The application of differential display is presented as a tool to identify genes induced by darkness or yellowing process in rice leaves.

Keywords: Gene expression; High throughput analysis; Differential display; RDA; Serial analysis; Expressed sequence tags

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1386207003331616

Publication date: 2000-06-01

More about this publication?

Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.