High Throughput Screening of Genetic Mutations/Polymorphisms by Capillary Electrophoresis

Author: Ren J.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 3, Number 1, February 2000 , pp. 11-25(15)

Publisher: Bentham Science Publishers

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Abstract:

Genetic mutations polymorphisms analyses play a great role in genetic and medical research, and clinical diagnosis. Most conventional methods for genetic assay are based on slab gel electrophoresis that is both labor-intensive and time-consuming. Recently, capillary electrophoresis (CE) has been used for genetic analysis instead of conventional slab gel electrophoresis. This technique can be automated and is characterized by short analysis time, small sample and reagents requirements, and high separation efficiency. CE has been successfully applied for mutation detection involving human tumor suppressor genes, oncogenes and disease-causing genes, and has shown a great potential for genetic mutation polymorphism screening of large numbers of clinical samples. In this article, an overview of the fundamental aspects of mutation/polymorphism assay methods in combination with CE is given and some key applications are summarized.

Keywords: High throughput screening; Geneti mutations; Polymorphisms; Capillary Electrophoresis; Human tumor suppressor genes; Onogenes; Disease causing genes; Ligase chain reaction; DNA sequencing; Hemochromatosis

Language: English

Document Type: Review article

DOI: 10.2174/1386207003327729

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