D-RNAi (Messenger RNA-antisense DNA Interference) as a Novel Defense System Against Cancer and Viral Infections

Authors: Lin S-L.; Ying S-Y.

Source: Current Cancer Drug Targets, Volume 1, Number 3, November 2001 , pp. 241-247(7)

Publisher: Bentham Science Publishers

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Abstract:

D-RNAi (Messenger RNA-antisense DNA interference), a novel posttranscriptional phenomenon of silencing gene expression by transfection of mRNA-aDNA hybrids, was originally observed in the effects of bcl-2 on phorbol ester-induced apoptosis in human prostate cancer LNCaP cells. This phenomenon was also demonstrated in chicken embryos and a human CD4+ T cell line, H9. The in vivo transduction of beta-catenin D-RNAi was shown to knock out more than 99 percent endogenous beta-catenin gene expression, while the in cell transfection of HIV-1 D-RNAi homolog rejected viral gene replication completely. D-RNAi was found to have long-term gene knockout effects resulting from a posttranscriptional gene silencing mechanism that may involve the homologous recombination between intracellular mRNA and the mRNA components of a D-RNAi construct. These findings provide a potential intracellular defense system against cancer and viral infections.

Keywords: D-RNAi Messenger RNA-antisense DNA Interference; Posttranscriptional gene; Silecncing ptgs

Language: English

Document Type: Review article

DOI: 10.2174/1568009013334151

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