Microarrays: Spotlight on Gene Function and Pharmacogenomics

Authors: Nees M.; Woosworth C.D.

Source: Current Cancer Drug Targets, Volume 1, Number 2, August 2001 , pp. 155-175(21)

Publisher: Bentham Science Publishers

Abstract:

The introduction of microarray technology has dramatically changed the way that researchers address many biomedical questions. DNA microarrays can measure expression of thousands of genes simultaneously, providing extensive information on gene interaction and function. Microarray technology is a powerful tool for identifying novel molecular drug targets and for elucidating mechanisms of drug action. Furthermore, microarrays can monitor the global profile of gene expression in response to specific pharmacologic agents, providing information on drug efficacy and toxicity. Over the last several years, dramatic advancements have occurred in array technology. In this review we describe basic aspects of microarray instrumentation and experimentation. Each of the major array formats including oligonucleotides arrays, spotted arrays, and macroarrays are examined, and advantages and options for using each format are presented. Important factors in the design and analysis of microarray experiments are also discussed. Most importantly, we explore recent developments in microarray technology that are relevant to pharmacogenomics and the discovery of gene function.

Keywords: Microarrays; post-genomic era; suppression techniques (SSH); Oligonucleotide Chips; Nylon Filter Arrays; Novel Array Technologies; Quackenbush; IMAGE consortium; immunophenotyping; Pathogenic Mechanisms; Cancer Genome Anatomy Project

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1568009013334197

Publication date: 2001-08-01

• Current Cancer Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular drug targets involved in cancer, e.g. disease specific proteins, receptors, enzymes, genes.
  Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics on drug targets involved in cancer.
  As the discovery, identification, characterization and validation of novel human drug targets for anti-cancer drug discovery continues to grow; this journal has become essential reading for all pharmaceutical scientists involved in drug discovery and development.

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• In this Subject: Oncology ,  Pharmacology
• By this author: Nees M. ;  Woosworth C.D.

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