Skip to main content

PMCA. A Decade of In Vitro Prion Replication

Buy Article:

$63.00 plus tax (Refund Policy)

Abstract:

Protein Misfolding Cyclic Amplification (PMCA) is a 10 years old in vitro technique based on a cyclic process leading to accelerate prion replication in vitro. The technique has been modified and adapted several times since its inception: new ideas, more sophisticated equipments and new applications have been essential elements for its upgrading. PMCA has proved to be an efficient method mimicking in vitro some of the fundamental steps involved in prion replication in vivo. Thus, it can be used to efficiently replicate a variety of infectious prion strains/species maintaining their strain specificity. It is an eminent technique for TSE diagnosis and is being used for detecting prions in blood in presymptomatic samples. On the other hand, the in vitro prion replication has been decisive to prove the protein only hypothesis, thanks to the generation of infectious prions by using substrates based exclusively on recombinant PrPC without any mammalian or synthetic co-factors. These achievements, in addition to the ability of PMCA for generating de novo prions in vitro as well as its use for evaluating the potential risks of different prion strains to humans and animals, make this technique as one of the most important tools from the last decade in the prion field.





Keywords: In vitro replication; PMCA; Transmissible Spongiform Encephalopathy (TSE); prion; scrapie

Document Type: Research Article

Publication date: September 1, 2010

More about this publication?
  • Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems.

    Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).

    Science at Chemistry-Biology Interface (Chemical informatics; Macromolecular catalysts and receptors; Enzymatic synthesis; Biosynthetic engineering; Combinatorial biosynthesis; Plant cell based chemistry; Bacterial and viral cell based chemistry; Chemistry of cellular processes in plants/animals; Receptor chemistry; Cell signaling chemistry; Drug design through understanding of disease processes; Synthetic biology; New high throughput screening techniques; Small molecular array fabrication; Chemical genomics; Chemical and biological approaches to carbohydrates proteins and nucleic acids design; Chemical and biological regulation of biosynthetic pathways; and Unnatural biomolecular analogs).
ben/ccb/2010/00000004/00000003/art00003
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more