Proteomic Analysis of Alzheimer's Disease Cerebrospinal Fluid from Neuropathologically Diagnosed Subjects

Authors: Maarouf, Chera L.; Andacht, Tracy M.; Kokjohn, Tyler A.; Castano, Eduardo M.; Sue, Lucia I.; Beach, Thomas G.; Roher, Alex E.

Source: Current Alzheimer Research, Volume 6, Number 4, August 2009 , pp. 399-406(8)

Publisher: Bentham Science Publishers

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Abstract:

A crucial need exists for reliable Alzheimer's disease (AD) diagnostic and prognostic tests. Given its intimate communication with the brain, the cerebrospinal fluid (CSF) has been surveyed intensively for reliable AD biomarkers. The heterogeneity of AD pathology and the unavoidable difficulties associated with the clinical diagnosis and differentiation of this dementia from other pathologies have confounded biomarker studies in antemortem CSF samples. Using postmortem ventricular CSF (V-CSF) pools, two-dimensional difference gel electrophoresis (2D DIGE) analyses revealed a set of proteins that showed significant differences between neuropathologically-diagnosed AD and elderly nondemented controls (NDC), as well as subjects with non-AD dementias. The 2D DIGE system identified a set of 21 different protein biomarkers. This panel of proteins probably reflects fundamental pathological changes that are divergent from both normal aging and non-AD dementias.

Keywords: Alzheimer's disease; cerebrospinal fluid; proteomics; DIGE; biomarkers

Document Type: Research article

DOI: http://dx.doi.org/10.2174/156720509788929318

Publication date: 2009-08-01

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Current Alzheimer Research publishes peer-reviewed frontier review and research articles on all areas of Alzheimer's disease. This multidisciplinary journal will help in understanding the neurobiology, genetics, pathogenesis, and treatment strategies of Alzheimer's disease. The journal publishes objective reviews written by experts and leaders actively engaged in research using cellular, molecular, and animal models. The journal also covers original articles on recent research in fast emerging areas of molecular diagnostics, brain imaging, drug development and discovery, and clinical aspects of Alzheimer's disease. Manuscripts are encouraged that relate to the synergistic mechanism of Alzheimer's disease with other dementia and neurodegenerative disorders. Book reviews, meeting reports and letters-to-the-editor are also published. The journal is essential reading for researchers, educators and physicians with interest in age-related dementia and Alzheimer's disease. Current Alzheimer Research provides a comprehensive 'bird's-eye view' of the current state of Alzheimer's research for neuroscientists, clinicians, health science planners, granting, caregivers and families of this devastating disease.

Proteomic Analysis of Alzheimer's Disease Cerebrospinal Fluid from Neuropathologically Diagnosed Subjects

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