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Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants

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Agrobacterium-mediated transient expression is a recently developed approach to quick and inexpensive largescale production of recombinant proteins in plant systems. In the course of this process, foreign gene expression occurs during several days after agroinfiltration without integration of recombinant DNA into plant genome and the level of target protein synthesis may be considerably higher than in stably transformed plants. This mini-review describes biological peculiarities of transient expression process, method development and optimization, and the range of application for biotechnological production of variable proteins. Patenting the transient expression protocols that mostly occurred during new millennium indicates the growing interest to this method as a perspective alternative to “classical” pro- and eukaryotic systems for recombinant protein production.

Keywords: Agrobacterium; Nicotiana benthamiana; plant; recombinant protein; transient expression; viral vector

Document Type: Research Article


Publication date: November 1, 2008

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  • Recent Patents on Biotechnology publishes review articles by experts on recent patents on biotechnology. A selection of important and recent patents on biotechnology is also included in the journal. The journal is essential reading for all researchers involved in all fields of biotechnology.

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