Mass Spectrometry, Proteomics, Data Mining Strategies and Their Applications in Infectious Disease Research.

Authors: Evangelou, Andreas; Gortzak-Uzan, Limor; Jurisica, Igor; Kislinger, Thomas

Source: Anti-Infective Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry - Anti-Infective Agents), Volume 6, Number 2, April 2007 , pp. 89-105(17)

Publisher: Bentham Science Publishers

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Abstract:

The ultimate goal of proteome research is the comprehensive description of all proteins present in a given sample using qualitative, quantitative and functional metrics. Traditionally, protein mixtures were first separated by twodimensional gel electrophoresis and spots of interest were excised, in-gel digested and analyzed by mass spectrometry (MS). In most cases, protein identification was done by MALDI-TOF-MS (matrix-assisted laser desorption/ionization time-of-flight). The methodology is time consuming and rarely leads to a comprehensive description of the analyzed proteome. Over the last years shot-gun expression profiling methodologies were developed and can identify thousands of proteins in complex biological samples in a single experiment. We will provide a short historic overview of proteome research and mass spectrometry technologies currently used in the systems biology community. In particular, we will summarize the developments and applications of shot-gun proteomics and allied computational data mining tools to medical research and infectious disease research.

Keywords: mass spectrometers (MS); high pressure liquid chromatography; electrospray ionization; MALDI-TOF-MS; Multidimensional Protein Identification Technology; Mass Spectral Peak Integration

Document Type: Research article

DOI: http://dx.doi.org/10.2174/187152107780361689

Affiliations: 1: Ontario Cancer Institute,Toronto Medical Discovery Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

Publication date: 2007-04-01

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Anti-Infective Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-infective agents.
Each issue contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in anti-infective medicinal chemistry.

Anti-Infective Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in anti-infective drug discovery.

Mass Spectrometry, Proteomics, Data Mining Strategies and Their Applications in Infectious Disease Research.

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