Electrochemical Sensor for Simultaneous Measurement of Nitrite and Superoxide Anion Radical Using Superoxide Dismutase-Mimetic Manganese(III) Tetrakis(1-methyl-4-pyridyl)Porphyrin on Polypyrrole Matrix
Abstract:An electrochemical highly sensitive nitrite (NO– 2 and superoxide anion radical (O•2 –) sensor was fabricated and developed based on an electrochemical deposition of manganese(III) tetrakis(1-methyl-4-pyridyl)porphyrin (MnTMPyP) in polypyrrole (PPy) matrix on Pt electrode. The surface morphological image of MnTMPyP-PPy and PPy matrix on Pt electrode was obtained by scanning electron microscopy exhibiting microporous structure. The electrochemical behavior of the MnTMPyP-PPy-Pt electrode as sensor investigated by cyclic voltammetry revealed that the characteristic of MnTMPyP reversible redox peaks obtained at –0.12 V and –0.38 V versus Ag/AgCl respectively. The sensor electrode showed an excellent electrocatalytic nitrite oxidase and superoxide dismutase (SOD) activities. This electrochemical sensor exhibited a linear current response over the concentration range from 0.8 to 1000 μM, with a detection limit of 0.8±0.03 μM for NO– 2 and a corresponding linear range from 0.6 to 1000 μM, with a detection limit of 0.6 ± 0.02 μM for O•2 –. In addition, the sensor displayed high sensitivity, good reproducibility and retained stability over a period of ∼4 weeks. This SOD-mimetic electrode was proved to be effective not only in detecting NO– 2 and O•2 – independently but also in determining the concentrations of NO – 2and O•2 – simultaneously in in vitro systems.
Document Type: Research Article
Publication date: October 1, 2011
More about this publication?
- The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites