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Indirect Dopamine Detection on a Glassy Carbon Electrode Based on Laponite/Tyrosinase

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A dopamine (DA) detection method based on the electrochemical reduction of dopaminechrome produced in the enzymatic reaction between DA and tyrosinase (PPO) is reported. Dopamine is monitored at –0.25 V versus Ag/AgCl (3 M KCl). The biosensor exhibits a good affinity to dopamine, Hill coefficient was found to be 1.12, the apparent Michaelis-Menten constant value for dopamine calculated from Lineweaver-Burk plot was 0.667 mM. The biosensor exhibits a high sensitivity (409.30 mA M–1 cm–2, a maximum current density of 240.56 μA cm–2 and a response time of 16 s. A wide linear range, 4 5 ·10–6 –4·10–4 M, and a low detection limit, 2.3 μM, were found.


Document Type: Research Article

DOI: http://dx.doi.org/10.1166/sl.2011.1715

Publication date: October 1, 2011

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