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An Amperometric Immunoassay for Human Chorionic Gonadotrophin Based on DNA-Multiwalled Carbon Nanotubes and Methylene Blue as Sensing Interface

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A new amperometric immunoassay based on Double-stranded calf thymus DNA dispersed multiwalled carbon nanotubes (MWNTs) and methylene blue (MB) was employed to develop a high sensitive immunosensor for determination of human chorionic gonadotrophin (HCG). DNA-MWNTs suspension was cast onto a glassy carbon electrode surface directly. Then methylene blue (MB) and gold nanoparticles were adsorbed onto modified electrode surface with the electrostatic interaction and chemosorption. At last, HCG antibody (anti-HCG) was immobilized onto the gold nanoparticles surface. The assembly process of the modified electrode was characterized by cyclic voltammetry and scanning electron microscope (SEM). The resulted immunosensor exhibited high sensitivity, broader measuring range from 0.1 to 10.0 and from 10.0 to 125.0 mIU/mL, with a low detection limit of 0.04 mIU/mL, good reproducibility and stability.
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