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Preparation and Characterization of Cadaverine Sensitive Nylon Threads

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Abstract:

We prepared catalytically active nylon threads, containing immobilized amine oxidase from pea seedlings for the application in biosensor systems as a selective biorecognition element for the detection of cadaverine (1,5-diaminopentane). The immobilization of the enzyme onto nylon threads, treated before enzyme binding consecutively with dimethyl sulfate and glutaraldehyde, resulted in threads with amine oxidase activity of (4.6 ± 0.4) × 10−4 IU/cm. The enzyme-containing threads enabled multiple and continuous measurements of cadaverine during 1 month with no detectable loss of activity. The unused threads retained their full activity at 4 °C for 3 months; after that date the activity began to decrease slowly. The KM values of the soluble and immobilized amine oxidase towards cadaverine were determined with an oxygen sensor and were 1.41 ± 0.17 mmol/l for soluble, and 0.60 ± 0.06 mmol/l for immobilized enzyme.

Keywords: AMINE OXIDASE; CADAVERINE BIOSENSOR; CATALYTIC PROPERTIES; COVALENT BINDING; IMMOBILIZATION; NYLON THREAD

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/sl.2009.1113

Publication date: August 1, 2009

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