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Preparing of Hybrid Bio-Organic–Inorganic Composite Membrane for Amperometric Mediatorless Glucose Micro Biosensor

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Abstract:

An amperometric micro glucose biosensor based on the hybrid bio-organic–inorganic composite sensitive membrane by which electrostatic absorption of glucose oxidase (GOD) in polyaniline (PAN) film on iridium oxide (Ir2O3) film electrode has been fabricated. The microelectrode exhibited a linear relationship with the concentration of glucose in the range of 0.01∼14 mM with a detection limit of 2.4 × 10−6 M. Iridium oxide electrode exhibits obviously electro-catalytic activity towards reduction of hydrogen peroxide (H2O2) at a low reductive potential. Ascorbic acid, uric acid and other oxidizable compounds coexisted (with glucose) in the sample did not cause interference to the determination. The selectivity, response time, reproducibility and lifetime of this micro biosensor are discussed. It has been used as a detector of flow-injection analysis (FIA) for glucose assay in blood serum directly.

Keywords: FLOW-INJECTION ANALYSIS; GLUCOSE BIOSENSOR; IRIDIUM OXIDE; MICROELECTRODE; POLYANILINE

Document Type: Research Article

DOI: https://doi.org/10.1166/sl.2009.1109

Publication date: 2009-08-01

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