Skip to main content

Detection of Mycobacterium tuberculosis Complex Using Surface Plasmon Resonance Based Sensors Carrying Self-Assembled Nano-Overlayers of Probe Oligonucleotide

Buy Article:

$113.00 plus tax (Refund Policy)


This paper describes the usage of a portable Surface Plasmon Resonance system for label free detection of DNA from Mycobacterium tuberculosis complex. A single-strand oligodeoxynucleotide, carrying a thiol end group (and also a spacer arm), which is complementary of the target characteristic sequence of Mycobacterium tuberculosis complex was used as the "probe" and immobilized onto the gold coated surfaces of the Surface Plasmon Resonance slides as Nano-overlayers by using a direct immobilization method based on the self-assembled over-layer formation. A modulator-spacer molecule 6-mercapto-1-hexanol was also co-immobilized in order to optimize the surface density of the probe-oligodeoxynucleotides and to orient them for better interaction. It is demonstrated that the reported Surface Plasmon Resonance biosensor platform can be used successfully to detect target molecules both synthetic and Polymerase Chain Reaction products very effectively at nM levels. The sensor platforms can also be regenerated with 2.5 mM HCl and reused effectively. It has been shown that developed biosensor has high specificity, sensitivity, sensor reproducibility and long shelf life for both small synthetic oligodeoxynucleotides and Polymerase Chain Reaction amplified real samples.


Document Type: Research Article


Publication date: 2009-08-01

More about this publication?
  • The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more