Detecting Penicillin in Milk with a Wireless Magnetoelastic Biosensor
Abstract:A wireless magnetoelastic biosensor for the direct detection of penicillin G in milk has been developed. The sensor was fabricated by applying a magnetoelastic ribbon with a layer of pH-sensitive polymer and upon it a sensing film containing bovine serum albumin (BSA) and penicillinase. The penicillinase-catalyzed hydrolysis of penicillin G decreases the solution pH, resulting in the polymer shrinking and consequently the resonance frequency of the magnetoelastic sensor increasing. Under optimum operating conditions the system provides a wide linear response between 1.9 mM and 5.0 mM with a very low detection limit of 1.3 mM. The immobilized penicillinase on magnetoelastic ribbon was very stable and provided good reproducible signal after regeneration up to 30 times with a relative standard deviation (R.S.D.) lower than 9%. Good recoveries and precisions were obtained when spiked raw milk samples were analyzed.
Document Type: Research Article
Publication date: 2009-02-01
More about this publication?
- The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites