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Single Cell Isolation on a Centrifugal Flow Disk with Integrated Tandem Microchambers

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Abstract:

Single cell analysis of various events in single cell requires quantitative measurement of associated factors to describe cellular function. However, such measurements are too difficult and time-consuming to perform using traditional techniques. We propose an advanced method for simultaneous isolation of a large number of cells using "tandem micro chambers" on a centrifugal flow disk. In order to realize the high throughput of single cell assay, we aim the common use of highly integrated micro chambers for the isolation and entrapment of a single cell reaction chambers in cell assay. With cell different in size such as microorganisms or Jurkat cell, single cell isolation for 40–200 particles/L was performed with the same disk and condition. Thus, the possibility that various kinds of cells which have different sizes would be isolated by our centrifugal flow disk was demonstrated by these results. The efficiency of single cell isolation was depended on Poisson distribution and the number of the isolated cells in this study was almost consistent with the theory.

Keywords: CENTRIFUGAL PUMPING; SINGLE CELL ISOLATION; TANDEM MICRO CHAMBER

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/sl.2008.540

Publication date: December 1, 2008

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