Development of the Potentiometric Lactate Biosensor Based on SnO2/ITO Glass Electrode
Abstract:In this study, a potentiometric lactate biosensor based on a SnO2/ITO glass electrode was fabricated with a simple enzyme immobilization process. The lactate dehydrogenase (LDH) and coenzyme (nicotinamide adenine dinucleotide, NAD+) were immobilized on the SnO2 sensing membrane using 3-glycidoxypropyltrimethoxysilane (GPTS). In addition, a solid-state lactate biosensor for potentiometric measurement was proposed. According to the experimental results, the sensitivity of the lactate biosensor based on the SnO2/ITO glass electrode was about 61.5 mV/decade between 6 mM and 40 mM lactate concentrations. Moreover, the relative response of the lactate biosensor maintained 84% of maximum response voltage during 25 times measurements. In application, the lactate biosensor showed obvious response voltage for detection in the dairy, and has advantages as low cost and easy fabrication. Therefore, the lactate biosensor based on the ITO glass electrode was successfully achieved in this study.
Document Type: Research Article
Publication date: December 1, 2008
More about this publication?
- The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites