Authors: Cioffi, N.; De Palo, F.; Calvano, C.D.; van der Werf, I.D.; Palmisano, F.; Zambonin, P.G.

Source: Sensor Letters, Volume 6, Number 4, August 2008 , pp. 654-661(8)

Publisher: American Scientific Publishers

Abstract:

Electrochemically synthesized nanoparticles (NPs) consisting of a gold core and a quaternary ammonium ("quat") shell were investigated as non-conventional matrix for the detection of low molecular weight analytes by Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry (MALDI-ToF-MS). The performance level of pristine NPs in promoting the analyte desorption/ionization processes was affected by the surfactant excess, due to shadowing of gold. Additional electrochemical protocols were developed for the controlled deposition of NPs on MALDI plates, aiming to improve the surface availability of nanostructured gold. Before use in mass spectrometry, the active area was treated by a potentiostatic procedure, that was proven to remove the excess of quat from the NPs surface. MALDI-MS and X-ray Photoelectron Spectroscopy showed that electrodeposited NPs can proficiently interact with small analytes, like cysteine and 4-fluoro-phenylalanine.

Keywords: GOLD NANOPARTICLE; MALDI; XPS; AMINO ACID DETECTION; PROTEOMICS

Document Type: Research article

DOI: http://dx.doi.org/10.1166/sl.2008.454

Publication date: 2008-08-01

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