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We report on the development of a new optical glucose sensor using o-dianisidine hydrochloride (DASD), glucose oxidase (GOD), and peroxidase (POD) immobilized onto a gold nanoparticle-modified Al2O3-based thin layer chromatography (TLC) sheet for D-glucose detection based on the colorimetric change in DASD. Images of the DASD/GOD/POD-modified site of the TLC plate demonstrated color changes at various D-glucose concentrations ranging between 0 and 10−1 M. The intensity increased with increasing D-glucose concentration, indicating that the D-glucose concentration can be visually estimated using the DASD/GOD/POD-modified site of the TLC plate. A plot of the logarithm values of D-glucose concentrations against the relative intensity showed good linearity, indicating that the D-glucose concentration could be determined based on the intensity of the images of the DASD/GOD/POD-modified site of the TLC plate.
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