Skip to main content

DNA Biosensors Prepared Using Platinum Nanoparticle-Deposited Glassy Carbon Electrodes

Buy Article:

$113.00 plus tax (Refund Policy)

Abstract:

DNA biosensors were prepared using platinum (Pt) nanoparticles that are electrochemically deposited on the surface of a glassy carbon (GC) electrode. The morphology of the surface of Pt nanoparticle-deposited GC electrodes was investigated with a scanning electron microscope (SEM). A cyclic voltammetry (CV) was also used for evaluating electrochemical performance of the Pt nanoparticle-deposited GC electrodes to establish the optimum conditions in electrochemical deposition of Pt nanoparticles. It was found that a highly sensitive electrode can be prepared by the deposition of Pt nanoparticles by applying step functions. A 19-mer ssDNA was immobilized on the Pt nanoparticle-deposited GC electrode and its electrochemical response to the complementary and three base-mismatched DNAs was studied using [Co(phen)3]3+ ion (phen = 1,10-phenanthroline) as an electrochemical indicator. The response of the Pt nanoparticle-deposited GC electrode is significantly higher as compared with that prepared based on a Pt disk electrode of same geometric surface area, suggesting a usefulness of the Pt nanoparticle-deposited electrodes. A linear calibration graph for the complementary DNA over the concentration of 2.1 × 10−9 – 2.1 × 10−7 mol/L was obtained and the limit of detection was 1.0 × 10−9 mol/L, while virtually no response was observed for the three-base-mismatched DNA. The DNA sensor can be used repeatedly over 8 times without deterioration.

Keywords: DNA BIOSENSOR; ELECTROCHEMICAL DEPOSITION; PLATINUM NANOPARTICLE

Document Type: Research Article

DOI: https://doi.org/10.1166/sl.2008.026

Publication date: 2008-02-01

More about this publication?
  • The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more