Detection of Lactose in Milk Samples Using a Wireless Multi-Enzyme Biosensor
Abstract:A wireless biosensor for analysis of lactose in milk samples has been developed by co-immobilizing -galactosidase (GLA), glucose oxidase (GOD), and catalase onto a magnetoelastic ribbon-like sensor which was pre-coated with a layer of pH-sensitive polymer. The GLA-catalyzed hydrolyzation of lactose produces -d-glucose, which was oxidized to gluconic acid under the catalysis of GOD, resulting in the pH-responsive polymer shrinking, and consequently the resonance frequency (fr) of the sensor increasing. The shifts in frequency were linearly proportional to the concentration of lactose from 2 mM to 16 mM with a detection limit of 0.72 mM. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) was applied to calibrate and confirm sensor measurements. The proposed wireless sensor can be extended for assays of lactose in sealed opaque containers.
Document Type: Research Article
Publication date: June 1, 2007
More about this publication?
- The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- ingentaconnect is not responsible for the content or availability of external websites