Authors: Nikitin, P.I.; Vetoshko, P.M.; Ksenevich, T.I.

Source: Sensor Letters, Volume 5, Number 1, March 2007 , pp. 296-299(4)

Publisher: American Scientific Publishers

Abstract:

New low-noise detection method has been developed and used for design of a new type of biosensors based on detection of nano-sized superparamagnetic particles or magnetic beads that serve as labels for biochemical reactions. The method is based on non-linear magnetization of such particles. The particles are exposed to a magnetic field having components at two frequencies f₁ and f₂. The response is measured at combinatorial frequencies f_i = m · f₁ + n · f₂, where m and n are integers (one of them can be zero). The integers can be varied to get the best signal-to-noise ratio, e.g., f_i = f₁ ±2 · f₂. Several readers have been designed for the particles counting and used for different immunoassay formats, including those compatible with immunoconcentration and magnetic enrichment of antigens. Registration of 0.1 ng/ml of Y. pestis antigen and 10³ cell/ml of Salmonella typhimurium has been demonstrated. The developed biosensing platforms can be used for medical diagnostics, points of care, food pathogen detection, water analysis, etc.

Keywords: BIOSENSORS; SUPERPARAMAGNETIC PARTICLES; NON-LINEAR MAGNETIC MATERIALS; DETECTION; COMBINATORIAL FREQUENCIES; ANTIGEN; ANTIBODY

Document Type: Research article

DOI: http://dx.doi.org/10.1166/sl.2007.007

Publication date: 2007-03-01

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