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A Copper Toxicity Biosensor Using Immobilized Cyanobacteria, Anabaena Torulosa

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Abstract:

A toxicity biosensor based on immobilized Anabaena torulosa for the determination of copper toxicity was developed. The live cyanobacteria cells were immobilized in a poly(2-hydroxyl ethyl methacrylate) (pHEMA) membrane and interfaced with an oxygen electrode. The analytical device allows the monitoring of cell inhibition due to copper toxicity through the changes of photosynthetic oxygen release. The 50% inhibitory response to Cu was between 0.5–0.7 mg/L Cu depending on the cell age. The linear response range to Cu was from 0.3–1.0 mg/L Cu (R2 = 0.9627). The whole-cell biosensor demonstrated rapid response (<5 mins) and the inhibition of photosynthetic activity was reproducible (RSD = 0–14%).

Keywords: INHIBITION; METAL TOXICITY; PHOTOSYNTHESIS; POLY(HYDROXYLETHYL METHACRYLATE); WHOLE-CELL BIOSENSOR

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/sl.2005.006

Publication date: January 1, 2005

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