Authors: Hanumegowda, Niranjan M.; White, Ian M.; Oveys, Hesam; Fan, Xudong

Source: Sensor Letters, Volume 3, Number 4, December 2005 , pp. 315-319(5)

Publisher: American Scientific Publishers

Abstract:

Simple and effective sensors for proteolytic activity are of interest to clinical diagnosis, homeland security, and nanofabrication. We demonstrate a novel label-free sensor for proteolytic activity utilizing an optical microsphere resonator that relies on circulation of the light to enhance the interaction with analytes. As a model, we employ the protease trypsin and a monolayer of BSA as a substrate on the sphere surface. We show a trypsin detection limit of approximately 10⁻⁴ Units/ml, corresponding to removal of 0.6% of each BSA molecule on the sphere surface. The detection limit obtained in this work indicates that a microsphere sensor with specificity for a protease of interest can be designed using synthesized peptides and a functionalized microsphere.

Keywords: SENSOR; WHISPERING GALLERY MODE; MICROSPHERE; RING RESONATOR; PROTEOLYTIC SENSOR; TRYPSIN

Document Type: Research article

DOI: http://dx.doi.org/10.1166/sl.2005.044

Publication date: 2005-12-01

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