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Novel Electrochemical Oral Biosensor for Histatin

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An amperometric biosensor for monitoring the level of saliva protein histatin is described. The biosensor-sensing elements comprise a layer of salivary histatin antibody self-assembled onto a gold quartz crystal by covalent attachment. The biospecific interaction between the immobilized histatin antibody and histatin antigen was monitored via a novel electroactive indicator, silver monodispersed on the gold surface. The silver is oxidized or reduced and the resulting peak currents provide analytical information about the concentrations of the salivary proteins. A detection limit of 0.626 pg/ml was obtained.
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Document Type: Research Article

Publication date: 2005-06-01

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