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Surface Patterning of Gram Positive and Gram Negative Bacterial Cells Using a Small Hydrophobic Molecule

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A facile novel approach for patterning Escherichia coli (a gram negative bacterium) and Bacillus subtilis (a gram positive bacterium) using a patterned template of Glycidyl 4-nonylphenyl ether (GNPE) is reported. Highly specific cell patterns with high cell-viability rates (over 85%) were obtained in each case. Cell coverage density was found to be proportional to GNPE concentration over identical surface areas. The versatility of the approach to immobilize both gram positive and gram negative bacterial cells using the same hydrophobic molecule highlights its potential in numerous cell array applications.
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Document Type: Research Article

Publication date: 2005-06-01

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