Skip to main content

Impedance-Probing of Mixed Amphiphile-Antibody Films Transferred onto Silver Electrodes

Buy Article:

$113.00 plus tax (Refund Policy)

Abstract:

The impedance spectroscopy was employed to follow the different steps of the immunosensor fabrication and the electronic transduction for the detection of the specific rabbit antigen IgG. A mixed amphiphile (octadecylamine)-antibody Langmuir-Blodgett (LB) film was structured at the air-water interface and transferred onto an octadecyl thiol (ODT) modified silver electrode. Impedance spectroscopy allows one to model the different layers with equivalent electrical circuits. A real probing of the ODT self-assembled monolayer (SAM) was done: electrical defects were found in the ODT SAM and they disappeared after deposition of the LBfilm. It can be suggested that these defects were suppressed by inclusion of octadecylamine (ODA) in the ODT layer. The total impedance of the mixed LBfilm increased in the presence of an increasing concentration of specific antigen. The equivalent circuit shows a decrease of the capacitance of the LBbilayer and an increase of its resistance. Such a structured building-up including biomolecules leads to a reproducible immunosensor, allowing detection of the specific antigen in the range from 10 ng ml –1 to 1200 ng ml –1.

Keywords: CAPACITANCE; EQUIVALENT CIRCUIT; IMMUNOSENSOR; LANGMUIR-BLODGETT FILM; OCTADECANETHIOL SAMS

Document Type: Research Article

DOI: https://doi.org/10.1166/sl.2004.056

Publication date: 2004-09-01

More about this publication?
  • The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more