Protein Array Patterning on Cyclic Olefin Copolymer (COC) for Disposable Protein Chip
Abstract:This paper presents a novel method of protein microarray patterning on Cyclic Olefin Copolymer (COC) in terms of surface modification for the development of a disposable protein chip. Protein microarrays have been generated via conventional photolithography and plasma deposition on the COC surface. The COC surfaces have been modified by the combination of O2, N2, and CF4 gases using RIE (Reactive Ion Etch) and their resultant surfaces have been characterized by contact angle. The protein (IgG, immunoglobulin G) adsorption on COC was evaluated by the intensities of anti-IgG conjugated to FITC (Fluorescein Isothiocyanate) or TRITC (Tetramethyl Rhodamine Iso-Thiocyanate). The absorption of IgG on plasma-modified surfaces having different contact angle was shown to be distinctive, which has been utilized for the protein patterning. In addition, the properties of COC were compared with those of other polymer materials, Polycarbonate (PC) and Polymethyl-Methacrylate (PMMA), which are also known as good material candidates for biochips. Keywords:
Document Type: Research Article
Publication date: September 1, 2004
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