Skip to main content

Protein Array Patterning on Cyclic Olefin Copolymer (COC) for Disposable Protein Chip

Buy Article:

$113.00 plus tax (Refund Policy)

Abstract:

This paper presents a novel method of protein microarray patterning on Cyclic Olefin Copolymer (COC) in terms of surface modification for the development of a disposable protein chip. Protein microarrays have been generated via conventional photolithography and plasma deposition on the COC surface. The COC surfaces have been modified by the combination of O2, N2, and CF4 gases using RIE (Reactive Ion Etch) and their resultant surfaces have been characterized by contact angle. The protein (IgG, immunoglobulin G) adsorption on COC was evaluated by the intensities of anti-IgG conjugated to FITC (Fluorescein Isothiocyanate) or TRITC (Tetramethyl Rhodamine Iso-Thiocyanate). The absorption of IgG on plasma-modified surfaces having different contact angle was shown to be distinctive, which has been utilized for the protein patterning. In addition, the properties of COC were compared with those of other polymer materials, Polycarbonate (PC) and Polymethyl-Methacrylate (PMMA), which are also known as good material candidates for biochips. Keywords:

Keywords: COC; CONTACT ANGLE; IMMUNOASSAY; PROTEIN ARRAY; SURFACE MODIFICATION

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/sl.2004.054

Publication date: September 1, 2004

More about this publication?
  • The growing interest and activity in the field of sensor technologies requires a forum for rapid dissemination of important results: Sensor Letters is that forum. Sensor Letters offers scientists, engineers and medical experts timely, peer-reviewed research on sensor science and technology of the highest quality. Sensor Letters publish original rapid communications, full papers and timely state-of-the-art reviews encompassing the fundamental and applied research on sensor science and technology in all fields of science, engineering, and medicine. Highest priority will be given to short communications reporting important new scientific and technological findings.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
asp/senlet/2004/00000002/F0020003/art00003
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more