Authors: Joong Hyun Kim; Jared P. Stephens; Dimitrios Morikis; Mihrimah Ozkan

Source: Sensor Letters, Volume 2, Number 2, June 2004 , pp. 85-90(6)

Publisher: American Scientific Publishers

Abstract:

A hybrid molecular beacon with inorganic fluorophore and organic quencher exhibits improved stability against photobleaching. To this end, inorganic colloidal quantum dots made of CdSe-ZnS with 3.7 nm diameter after surface modification are attached to the 5 end of molecular beacons (MBs) to replace organic-based fluorophores. The quenching mechanism (fluorescence resonance energy transfer (FRET)) for this hybrid arrangement is discussed and supported with 3-dimensional molecular modeling. In addition, a covalent bond between quantum dot and molecular beacon is verified together with the specificity of the hybrid molecular beacon. Finally, a comparison of the hybrid molecular beacon with its organic-based counterpart (6-FAM, which has close emission characteristics to the used quantum dot) shows a more stable lifetime in the case of hybrid arrangement.

Keywords: MOLECULAR BEACON; HYBRID; QUANTUM DOT; PROBE; DNA

Document Type: Research article

DOI: http://dx.doi.org/10.1166/sl.2004.042

Publication date: 2004-06-01

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