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Antioxidant Effect of Gold Nanoparticles Synthesised from Curcuma Longa Restrains 1-Methyl-2-Phenyl Pyridinium Ion Induced Stress in PC12 Cells

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Abstract:

The green synthesis of nanoparticles biologically inspired due to the challenges faced by chemical synthesis protocol of nanoparticles in being toxic, flammable and unstable and is expected to open new avenue to fight and prevent diseases. In this study we report the synthesis of gold nanoparticles from Curcuma longa root extract by 'exploiting' the reduction capabilities of varied phytochemicals present in it and was confirmed by FTIR, UV-Visible pectroscopy and TEM that interpreted the formation of gold nanoparticles by the reduction of gold salts. We extended our research to utilize the efficacy of these synthesized biocompatible gold nanoparticles (Tu-AuNPs) as an antioxidant against 1-methyl-2-phenyl pyridinium ion (MPP+) induced cytotoxicity and cell death in PC-12 cells. Incubation of PC-12 cells with Tu-AuNPs prevented MPP+ -induced loss in cell viability and enhanced LDH leakage in a dose-dependent manner. In addition, reduction in the level of non-protein thiol, glutathione (GSH); activities of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) as well as the increased MDA levels have also been found to be prevented by this nanoparticles. Moreover, MPP+ exposure caused inhibition of complex I activity. In addition, MPP+ introduces apoptosis as the primary phenomena of cell death as evidenced by DAPI staining, flow cytometric analyses. Tu-AuNPs treatment, however, counteracted these changes and maintains normalcy in PC-12 cells. Our results specify that Tu-AuNPs possesses cytoprotective activity against MPP+-induced oxidative cellular damage and prevents PC-12 cells from apoptotic death. Taken together, the above results suggest that gold nanoparticles from Curcuma longa root extract may be a candidate drug for the treatment of oxidative stressinduced neurodegenerative disease.

Keywords: GOLD NANOPARTICLES; OXIDATIVE STRESS; PARKINSON'S DISEASE; TURMERIC

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jns.2012.1018

Publication date: April 1, 2012

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  • Recent advances in nanomaterials indicates that the central nervous system (CNS) is susceptible to nanoparticle induced alterations leading to functional or structural alterations. This knowledge is currently disseminated in vast array of journals dealing with broad subject areas related to pharmacology, toxicology, neuroscience or nanosciences. Thus, there is an urgent need to collect all these diverse information related to nanoscience and brain function in one place using Journal of Nanoneuroscience for the benefit of the scientific community, researchers, health planners, health care providers, policy makers, environmentalists, biologists, chemists, and physicist in this emerging area of medical science.
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