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Comparative Study on Antimicrobial Property of Silver Nanoparticles Synthesized by Fusarium Equiseti and Fusarium Solani

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Abstract:

There is an urgent need to develop alternate antimicrobial agents and huge amount of research is underway for the search for a novel antimicrobial compounds. With increase in microbes acquiring resistance to multiple antibiotics, an alternate stratergy has to be developed. The new development in the field of nanotechnology is expected to influence the field of therapeutics. The objective of the present investigation was to synthesize silver nanoparticles by fungal cultures under stress condition, evaluate, and compare its antimicrobial property on different bacterial pathogens. Production of nanoparticles using biological systems has gained more importance in the present scientific world. Four different fungal colonies were isolated from coalmine soil sample and cultured in Potato Dextrose Agar medium, Sabouraud's Dextrose Agar and Rose Bengal Agar. Among which Fusarium equiseti and Fusarium solani were chosen for synthesis of silver nanoparticles. Morphological identification and 18S r RNA gene sequencing was conducted. The brown colouration of the culture broth after 3–4 d indicates the production of silver nanoparticles. The silver nanoparticles produced were analyzed by UV-Vis Spectrophotometer, X-ray Diffraction (XRD), Atomic Force Microscopy (AFM) and screened for the antimicrobial property against pathogens. A comparative study of their antimicrobial property was performed.

Keywords: AFM; ANTIMICROBIAL ACTIVITY; SILVER NANOPARTICLES; UV-VIS; XRD

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbns.2012.1064

Publication date: June 1, 2012

More about this publication?
  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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