Synthesis, Characterization and In Vitro Study of Curcumin-Functionalized Citric Acid-Capped Magnetic (CCF) Nanoparticles as Drug Delivery Agents in Cancer

Authors: Wani, Kirtee D.; Kitture, Rohini; Ahmed, Anansa; Choudhari, Amit S.; Koppikar, Soumya J.; Kale, Sangeeta N.; Kaul-Ghanekar, Ruchika

Source: Journal of Bionanoscience, Volume 5, Number 1, June 2011 , pp. 59-65(7)

Publisher: American Scientific Publishers

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Abstract:

Curcumin, a naturally occurring polyphenol extracted from turmeric (Curcuma longa), an indispensable culinary spice in Indian diet, has been known to function as a potent anticancer agent. However, its limited solubility and absorption into body tissues limits its bioavailability. Magnetic nanoparticles, typically Fe3O4, have been shown to possess various biomedical applications including hyperthermia, MRI, magnetic separation and targeted drug delivery. In the present paper, we report the synthesis of a highly aqueous stable suspension of citric acid (CA) capped iron oxide (Fe3O4) nanoparticles conjugated to curcumin (CU). The significance of this report lies in the use of citric acid as a capping agent for magnetic nanoparticles in combination with curcumin. Citric acid has been mainly used to overcome the problem of limited bioavailability of curcumin as well as to impart ferrofluid property to the nanoparticles. The Curcumin-Citric acid-Fe3O4 nanoparticles (CCF) were duly characterized by X-ray diffraction (XRD) and conjugated nanoparticles were confirmed by Fourier transform infrared spectroscopy (FTIR). The percent drug loading was evaluated by thermo gravimetric analysis (TGA). CCF nanoparticles were studied in vitro to determine whether the curcumin retained its anticancer activity after conjugation with citric acid capped nanoparticles. In vitro uptake of CCF nanoparticles into breast cancer cell line, MCF7, was confirmed by Prussian blue staining. MTT assay was performed to determine cytotoxicity of the nanoparticles towards MCF7. Growth assays proved that the anticancer activity of curcumin was retained even after conjugation with CA.

Keywords: ANTICANCER; CITRIC ACID; CURCUMIN; MAGNETIC NANOPARTICLES

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbns.2011.1041

Publication date: June 1, 2011

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  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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