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Extracellular Biosynthesis of Silver Nanoparticles Using Aqueous Extract of Candida viswanathii

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Abstract:

One of the important areas of nanotechnology is the development of reliable processes for the synthesis of nanomaterials over a range of sizes with good monodispersity. Present work describes an eco-friendly method for the synthesis of silver nanoparticles by cell free extract (CFE) of Candida viswanathii. Parameters like culture age (stationary phase growth), cell mass concentration (500 mg/ml), temperature (37 °C) and reaction time (12 h) have been optimized for the maximum nanoparticle synthesis. Nanoparticle formation was confirmed by UV-Vis spectroscopy and EDX (energy dispersive X-rays) analysis, size and morphology was determined by Transmission electron microscopy and particle size distribution was assessed by zeta sizer. Synthesized nanoparticles lie in the size range of 30–40 nm with high negative zeta potential value of –31 mV indicating excellent stability. Maximum amount of nanoparticle was produced in a reaction mixture containing cell free extract from a cell mass of 500 mg/ml at 37 °C in 12 h.

Keywords: CANDIDA VISWANATHII; CELL FREE EXTRACT; NANOPARTICLE CHARACTERISATION; SILVER NANOPARTICLES

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbns.2011.1040

Publication date: June 1, 2011

More about this publication?
  • Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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