@article {Seshadri:2011:1557-7910:18,
title = "Deoxyribonucleic Acid Functionalized with Gold Nanoparticles: A Golden Route to Molecular Biology",
journal = "Journal of Bionanoscience",
parent_itemid = "infobike://asp/jobn",
publishercode ="asp",
year = "2011",
volume = "5",
number = "1",
publication date ="2011-06-01T00:00:00",
pages = "18-25",
itemtype = "ARTICLE",
issn = "1557-7910",
url = "https://www.ingentaconnect.com/content/asp/jobn/2011/00000005/00000001/art00002",
doi = "doi:10.1166/jbns.2011.1044",
keyword = "DNA, HYBRIDIZATION, GOLD NANOPARTICLES",
author = "Seshadri, Sachin and Kowshik, Meenal",
abstract = "Understanding the molecular processes such as DNA replication, transcription, translation and gene regulation has been the focus of biological research for several decades. Abberations in the above processes form the basis of several diseases and hence their study is of paramount importance.
Gold nanoparticles (AuNPs) possess several unique properties, however it is their surface plasmon resonance (SPR) in the 520550 nm region that make them ideal candidates for molecular biology research. Several assays are based on the red-purple color change observed upon AuNP aggregation.
The DNA is a unique biomolecule in its ability to replicate, bind to complementary strands and show sequence-specific interactions with proteins. Using several functionalization chemistries, AuNP surfaces have been coated with DNA strands. This simple construct has been used as a gene probe
and more recently to study DNA-protein interactions. Optimization of parameters such as particle size, coverage of DNA strands on the AuNP surface, length of the DNA has attracted a lot of attention recently to push the boundaries of sensitivity of the DNA-AuNP based assays. These assays are
simple and procedures such as gel electrophoresis, visual detection or microscopy are the prime requisites in almost all cases. In this review, we present the applications of DNA functionalized with AuNPs in molecular biology and various strategies underlying them.",
}