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Protein Detection at Zeptomole Quantities: A Nanoparticle Based Immunoassay Using Total Internal Reflection Fluorescence Microscopy
Authors: Larsen, Brian A.; Hamilton, Benjamin D.; Stoldt, Conrad R.
Source: Journal of Bionanoscience, Volume 2, Number 2, December 2008 , pp. 119-123(5)
Publisher: American Scientific Publishers
Abstract:
An immunoassay using total internal reflection fluorescence (TIRF) microscopy was developed to detect zeptomole quantities of protein. This approach was demonstrated using cancer antigen 125 (CA125), an ovarian cancer associated oncoprotein, as the analyte, and an anti-CA125 monoclonal antibody pair to capture and label the oncoprotein with a fluorescent nanoparticle. The captured and labeled analyte was imaged using TIRF microscopy as individual fluorescent binding events, enabling quantification of <1 zmol of CA125 with an intra-assay precision within ±10%. The low detection limit of this method demonstrates its potential utility as a tool for the proteomics of single cell protein quantities.Keywords: PROTEIN ASSAY; NANOPARTICLE; SINGLE MOLECULE DETECTION; TIRF
Document Type: Short communication
DOI: http://dx.doi.org/10.1166/jbns.2008.036
Publication date: 2008-12-01
- Bionanoscience attempts to harness various functions of biological macromolecules and integrate them with engineering for technological applications. It is based on a bottom-up approach and encompasses structural biology, biomacromolecular engineering, material science, and engineering, extending the horizon of material science. The journal aims at publication of (i) Letters (ii) Reviews (3) Concepts (4) Rapid communications (5) Research papers (6) Book reviews (7) Conference announcements in the interface between chemistry, physics, biology, material science, and technology. The use of biological macromolecules as sensors, biomaterials, information storage devices, biomolecular arrays, molecular machines is significantly increasing. The traditional disciplines of chemistry, physics, and biology are overlapping and coalescing with nanoscale science and technology. Currently research in this area is scattered in different journals and this journal seeks to bring them under a single umbrella to ensure highest quality peer-reviewed research for rapid dissemination in areas that are in the forefront of science and technology which is witnessing phenomenal and accelerated growth.
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- In this Subject: Engineering/Technology , Technology , Physics (General)
- By this author: Larsen, Brian A. ; Hamilton, Benjamin D. ; Stoldt, Conrad R.
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