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Naked Eye Detection of Salmonella typhimurium Using Scanometric Antibody Probe

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Salmonella is one of the most common foodborne pathogens, and Salmonella outbreaks are mostly associated with the intake of contaminated food or drink. Therefore, the rapid and sensitive on-site detection of Salmonella is very important. We report a naked eye detection method for Salmonella typhimurium using scanometric antibody probe. The antibody-attached glass substrate was treated with Salmonella typhimurium and the scanometric antibody probe was applied. After Ag enhancement of the probe, Salmonella typhimurium could be detected with the naked eye. The scanometric antibody probe was prepared by simply mixing Au nanoparticles, gold binding peptide-protein G, and antibody against Salmonella typhimurium. This probe can act as a signal enhancer and thus allows for an extremely simple, rapid, and efficient analysis of Salmonella typhimurium by the naked eye. We detected Salmonella typhimurium at a low concentration of 103 CFU/ml and clearly distinguished this bacterium from other foodborne pathogens. Furthermore, we successfully detected Salmonella typhimurium in milk, suggesting that this method can be useful in real-life samples. Because the scanometric antibody probe can be expanded to various types of antibodies, this naked eye detection method could be employed for the detection of various types of pathogens.
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Keywords: Antibody; Gold Binding Peptide; Naked Eye; Protein G; Salmonella

Document Type: Research Article

Affiliations: 1: Hazards Monitoring Bionano Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea 2: Hazards Monitoring Bionano Research Center, Korea Research Institute ofBioscience and Biotechnology, Daejeon 34141, Korea 3: Department of Chemistry, Korea Advanced Institute ofScience and Technology, Daejeon 34141, Korea 4: Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea

Publication date: 2017-07-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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