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Comparative Physicochemical Characterization and Bioavailability of Nano-Liposomes Formed by Mixing Lipids at Different Ratios

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Liposomes are small lipid vesicles that mimic biological membranes and have been spotlighted in the clinical field due to their ability to enclose a biologically active substance of any structure and to release it into the host’s body. This study compares the physicochemical properties and biological activity of nano-liposomes with different compositions to determine the most effective formulation for further in vivo application. Nano-scale liposomes composed of different ratios of 1,2-dimyristoylsn-glycero-3-phosphoethanolamine (DMPE), dihexadecyl phosphate (DCP), and cholesterol (Chol): DMPE, DMPE/DCP, DMPE/Chol, and DMPE/DCP/Chol were produced. The thermal phase transition was assessed via differential scanning calorimetry (DSC); the particle size, via dynamic light scattering (DLS); the colloidal stability, via the zeta potential; the direct morphological characterization, via transmission electron microscopy (TEM); and the protein encapsulation efficiency. The bioavailability was also investigated with respect to the immunological responses via porcine interferon gamma (IFN-γ) enzyme-linked immunospot (ELISPOT) assay in peripheral blood mononuclear cells (PBMC) of immunocompetent pigs. All the liposomes can be expected to be stable in an in vivo physiological temperature, and the liposomes that were prepared from DMPE/DCP showed the best efficiency in the in vitro model that mimicked the release of a bioactive substance in vivo. In the result of DLS and the zeta potential for the investigation of the colloidal stability in the system, DMPE/DCP/Chol appeared better than the other formulations. The porcine IFN-γ ELISPOT assay results postulated that DMPE/DCP most potently induced the IFN-γ secretion by PBMC, followed by DMPE/DCP/Chol and DMPE alone, in that order.
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Document Type: Research Article

Publication date: 2012-07-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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