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A Convenient Alcohol Sensor Using One-Pot Nanocomposite Entrapping Alcohol Oxidase and Magnetic Nanoparticles as Peroxidase Mimetics

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A colorimetric biosensor for convenient quantification of ethanol and methanol is described. The biosensor utilizes a ‘one-pot’ nanocomposite consisting of Fe3O4 magnetic nanoparticles (MNPs) and alcohol oxidase (Al O×) simultaneously entrapped in large pore sized mesocellular silica. Al O× immobilized in the silica generates H2O2 in the presence of alcohol in a sample, which subsequently activates MNPs in the mesopores of the silica to convert a colorimetric substrate into a colored product. Using this strategy, a target alcohol was specifically detected through a very convenient colorimetric signal resulting from the combined reactions. This strategy enabled successful sensing of ethanol and methanol in a linear concentration range from 100 to 500μM with a detection limit as low as 25μM by employing 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) as a pero×idase substrate. Along with e×cellent reusability via simple magnetic capturing, enhanced operational stability was achieved by the nanocomposite system. The present nanocomposite would serve as a novel platform for rapid and convenient analysis of alcohol.

Document Type: Research Article


Publication date: 2012-07-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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