Fabrication of Non-Enzymatic Biosensor Based on Metallic Catalyst-TiO2 Hollow Sphere Nanocomposite for Determining Biomolecules
Abstract:A PtRu@Tio2-hollow nanocomposite for the detection of biomolecules was synthesized by chemical reduction. First, poly(styrene-co-vinylphenylboronic acid), PSB, was prepared as a template (∼250 nm) by surfactant-free emulsion polymerization. Second, PSB/Tio2 core–shell spheres were prepared by sol–gel reaction. Finally, Tio2 hollow spheres (Tio2–H) were then formed after removing the PSB template by calcination at 450 °C under air atmosphere. To prepare the electrocatalyst, PtRu nanoparticles (NPs) were deposited onto the Tio2–H surface by chemical reduction. The prepared PtRu@Tio2–H nanocomposite was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and elemental analysis. A non-enzymatic sensor was fabricated by depositing the as-prepared PtRu@Tio2–H nanocomposite on the surface of a glassy carbon electrode (GCE), which was prepared by a hand casting method with Nafion solution as a binder. The sensor was tested as a biomolecule sensor, especially for the detection of glucose and dopamine. The cyclic voltammograms (CV) obtained during the oxidation studies revealed that the PtRu@Tio2–H nanocomposite showed better catalytic function toward the oxidation of dopamine. The sensing range of the non-enzymatic sensor for glucose was 5.0–100 mM in a phosphate buffer. The results demonstrated the potential usefulness of this bimetallic@Tio2–H bifunctional catalyst for biosensor applications.
Document Type: Research Article
Publication date: 2012-07-01
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