If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Cell Chip to Detect Effects of Graphene Oxide Nanopellet on Human Neural Stem Cell

$113.00 plus tax (Refund Policy)

Buy Article:

Abstract:

The graphene oxide (GO) nanopellet, a potentially useful carbon-based material, recently started being applied in cell-based research areas. Its toxicity assessment using the neural-stem-cell-based chip has not been thoroughly reported yet, though. Herein, a cell chip was fabricated to electrochemically detect the toxic effects of GO nanopellets on HBI.F3 cells. The RGD peptide was immobilized on the gold electrode surface to enhance the binding affinity of the HBI.F3 cells to the electrode surface. A clear redox peak appeared when the HB1.F3 cells were analyzed via cyclic voltammetry. The GO nanopellet was analyzed via Raman spectroscopy to confirm its distinct structural characteristics that normally differ from those of graphite oxide. After GO was added to the HB1.F3 cells, differential pulse voltammetry was performed to discover the toxic effects of GO nanopellets on HB1.F3 cells. A negative correlation was achieved between the concentration of the GO nanopellets and the cell viability, which was verified via both MTT assay and a microscopic imaging tool. Thus, these electrochemical tools can be usefully applied to the toxicity assessment of various kinds of carbon-based materials.

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2012.6378

Publication date: July 1, 2012

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more