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Assessment of Liposome Biodistribution by Non-Invasive Optical Imaging: A Feasibility Study in Tumour-Bearing Mice

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Abstract:

Liposomal encapsulation of cytostatics improves drug delivery to tumour tissue and reduces dose-limiting systemic toxicities. Development and evaluation of new liposome formulations is time consuming and costly with high demands for experimental animals. A faster and less demanding means of comparing several product candidates may be provided by use of non-invasive methods for assessing pharmacokinetics and biodistribution. In this study we have evaluated the feasibility of using small animal fluorescence optical imaging as a strategy to study liposome accumulation in tumours. Liposomal doxorubicin (Caelyx®) was labelled with a lipophilic carbocyanine tracer and administered to tumour-bearing mice. Subsequently, the in vivo distribution of the labelled liposomes was followed over time by fluorescent optical imaging. The results revealed a gradual increase in tumour fluorescence, indicating accumulation of the liposomes reaching plateau levels at 48 h post injection. However, due to loss of dye from liposomes during circulation combined with substantial scattering and absorption of in vivo fluorescent signal, reliable quantitative correlation between the biodistribution profile of the labelled liposomes and doxorubicin could not be obtained.

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2012.5852

Publication date: 2012-03-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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