Cytotoxicity and TNF-α Secretion in RAW264.7 Macrophages Exposed to Different Fullerene Derivatives

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Abstract:

Fullerene derivatives have been reported as potential nanomedicines, however the role of surface chemical modification on the biological effects remains unclear. In this study five kinds of water soluble C60 derivatives with different surface chemical modification, C60-(OH)20 (HFD), C60-(β-Ala)10.1 (AFD), C60-(Lys)8.7 (KFD), C60-(Arg)8.6 (RFD) and C60-(NH(CH2)2NH2)8.8 (NFD) were synthesized. Their cytotoxicity as well as TNF-α secretions were evaluated in RAW264.7 macrophage cell line. The results show that no significant cytotoxicity can be observed upon 24 h exposure to C60 derivatives at less than 50 μg/mL However, higher concentration (>100 μg/mL) of these C60 derivatives decreases the proliferation of RAW264.7. The cytotoxicity of these fullerene derivatives is probably through the apoptosis pathway, while the extent of cytotoxicity varies with the different surface charges. Higher celluar uptake of HFD was observed in RAW264.7 cells than AFD, which correlates with the more toxic effect of HFD over AFD. The secretion of cytokine tumor necrosis factor alpha (TNF-α) was determined to evaluate the immunostimulating activity of these fullerene derivatives. The data show that the fullerene derivatives with negative surface charges secrete more TNF-α, whereas derivatives with positive charges show insignificant effect. The possible influence of various surface charge property on the observed biological effects is discussed.

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2012.5681

Publication date: March 1, 2012

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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