If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

2,4,6-Tris (2-pyridyl)-1,3,5-triazine Nanobelts as an Effective Fluorescent Sensing Platform for DNA Detection

$113.00 plus tax (Refund Policy)

Buy Article:

Abstract:

In the present study, we report on the use of 2,4,6-tris (2-pyridyl)-1,3,5-triazine nanobelts (TPTNBs) as an effective fluorescent sensing platform for DNA detection for the first time. The general concept used in this approach is based on adsorption of fluorescently labeled single-stranded DNA (ssDNA) probe by TPTNB, due to the strong ππ stacking between unpaired DNA bases and TPTNB. As a result, the fluorophor is brought into close proximity of TPTNB, leading to fluorescence quenching. Upon presence of the target ssDNA, specific hybridization with the target takes place to form a double-stranded DNA (dsDNA). The resultant dsDNA cannot be adsorbed by TPTNB due to its rigid conformation and the absence of unpaired DNA bases. Thus, the fluorophor is seperated from TPTNB accompanied by fluorescence recovery. The present system shows a detection limit as low as 3 nM and has a high selectivity down to single-base mismatch.

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2012.5740

Publication date: March 1, 2012

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more