Binding Studies of L-Tryptophan to Human Serum Albumin with Nanogold-Structured Sensor by Piezoelectric Quartz Crystal Impedance Analysis
Authors: Long, Yumei; Yao, Shouzhuo; Chen, Jinhua
Source: Journal of Nanoscience and Nanotechnology, Volume 11, Number 12, December 2011 , pp. 11074-11078(5)
Publisher: American Scientific Publishers
Abstract:Nanogold-modified sensor was constructed and applied to study the binding of L-tryptophan to human serum albumin (HSA)in situ by piezoelectric quartz crystal impedance (PQCI) analysis. It was interesting that the as-prepared nanogold modified sensor was more sensitive and biocompatible than bare gold electrode. The frequency changes due to protein adsorption on the nanogoldmodified sensor might be described as a sum of two exponential functions and detailed explanation was given. Additionally, the kinetics of the binding process was also investigated. The binding constant (K) and the number of binding site (n) for the binding process without competitor are fitted to be 1.07 × 104 (mol l−1)−1 s−1 and 1.13, respectively, and 2.24 × 103 (mol l−1)−1 s−1 and 1.18, respectively for the binding process with competitor.
Document Type: Research Article
Publication date: December 2011
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