Synthesis and Characterization of Fe3O4@SiO2 Core–Shell Magnetic Microspheres for Extraction of Genomic DNA from Human Whole Blood

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Abstract:

An improved procedure was described for preparation of monodisperse Fe3O4@SiO2 core–shell magnetic microspheres via solvothermal method followed by a modified Stöber process. The magnetic composite microspheres were characterized with TEM, SEM, FTIR and VSM. Genomic DNA was then extracted from human whole blood using the as synthesized magnetic microspheres on the Eppendorf epMotion5075 workstation. The quality of eluted DNA was evaluated by PCR. The method was very expeditious without using toxic compounds such as phenol or chloroform and can be used for further molecular biology experiments.

Keywords: CORE-SHELL; DNA EXTRACTION; HUMAN WHOLE BLOOD; MAGNETIC MICROSPHERES

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2011.5200

Publication date: December 1, 2011

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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