Skip to main content

Carboxymethylated Dextran-Coated Magnetic Iron Oxide Nanoparticles for Regenerable Bioseparation

Buy Article:

$105.00 plus tax (Refund Policy)

Carboxymethylated dextran (CMD)-coated magnetic iron oxide nanoparticles (MNPs) were synthesized using a co-precipitation method. Compared to neutral dextran coated MNPs, the CMD coating provides good dispersity and colloidal stability to the CMD-MNPs. In particular, the carboxyl groups on the CMD can be readily activated for covalent attachment of antibody molecules. The superparamagnetic property of the antibody-covered CMD-MNPs enables the captured antigen to be separated from the sample solution and CMD coating significantly reduces the nonspecific binding of the nanoparticles. Regeneration of the anti-BSA antibody-covered CMD-MNPs with NaOH does not significantly decrease the antibody activity, and the repeated magnetic separation and washing steps cause only small loss of the starting materials. The method was found to be highly reproducible (RSDs for BSA adsorption and desorption are between 0.78% and 5.1%). The anti-BSA antibody-covered CMD-MNPs possess good selectivity and are able to capture protein antigens from real samples.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: BIOSEPARATION; CARBOXYMETHYLATED DEXTRAN; MAGNETIC NANOPARTICLES; SURFACE MODIFICATION

Document Type: Research Article

Publication date: 2011-11-01

More about this publication?
  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more