Preparation of Core–Shell ZnO-SiO2 Nanowires–Nanotubes for Immobilization of the Alkaline Protease Enzyme
The main goal of enzyme immobilization is industrial re-use of enzymes for many reaction cycles. In this purpose, simplicity and improvement of the enzyme properties have to be strongly associated with the design of protocols of enzyme immobilization. In the last decade, nanosized materials have been widely used as a support for enzyme immobilization, for instance, silica nanotubes, phospholipid bilayers, self-assembled monolayers Langmuir_Blodgett films, polymer matrices, galleries of α-zirconium, phosphate, mesoporous silicates such as MCM-41, silica nanoparticles. In this work, we report synthesis of core shell ZnO/SiO2 nanowires and used them as a support for immobilization of the alkaline protease. Characterization of this assembled systems was carried out by, Energy-dispersive X-ray spectroscopy (EDAX), Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM). Biocatalytic activity of the alkaline protease in this bioconjugate system was examined and the results showed an increase of biocatalytic activity, in comparison with the free enzyme in solution.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
Document Type: Research Article
Publication date: 2011-10-01
More about this publication?
- Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites